The nature of the phosphate complex of sulphite oxidase from electron-paramagnetic-resonance studies.
نویسندگان
چکیده
The phosphate complex of sulphite oxidase in the Mo(V) oxidation state was investigated by e.p.r. spectroscopy. Third-derivative spectra reveal a wealth of structural detail previously unobserved in this spectrum. Most notable is the presence of hyperfine coupling from two inequivalent I = 1/2 nuclei, which we tentatively attribute to two 31P nuclei. Unresolved hyperfine interactions from at least one exchangeable 1H nucleus are also present.
منابع مشابه
electron-paramagnetic-resonance studies using oxygen-17
Studies of the effect of substitution with 170 on the e.p.r. spectra at 9 and 35 GHz of Mo(V) in the phosphate complex of sulphite oxidase are reported. Substitution of '7O-enriched water for normal water, for samples of the enzymes reduced by sulphite in the presence of normal phosphate, produced no detectable effect on the e.p.r. signal. If phosphate substituted with 170 was used, coupling du...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 256 1 شماره
صفحات -
تاریخ انتشار 1988